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KMID : 0861020200350040017
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Korea Journal of Herbology
2020 Volume.35 No. 4 p.17 ~ p.23
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Effect on rat model of reflux esophagitis treated with Charybdis japonica extract
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Nam Hyeon-Hwa
Seo Yun-Soo
Lee Ji-Hye
Seo Young-Hye
Yang Sun-Gyu
Moon Byeong-Cheol
Kim Wook-jin
Nan Li
Choo Byung-Kil
Kim Joong-Sun
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Abstract
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Objectives : In this study, we investigated the protective effects of Charybdis japonica (C. japonica) water extract on the acute reflux esophagitis in rat models.
Methods : Twenty rats were divided into four groups for examination: normal control group (n=6), the reflux esophagitis group (n=6), reflux esophagitis treated with positive control group (ranitidine 40 ㎎/㎏, n=6), reflux esophagitis treated with C. japonica group (100 ㎎/㎏, n=6). All rats fasted for 18 hr and then were induced with reflux esophagitis by a pylorus and forestomach ligation operation. After 4 hr, the rats were sacrificed. The pro- inflammatory cytokine and proteins expression measured by western bolt assay, and the histopathological analysis of the esophageal mucosa measured by hematoxylin and eosin staining.
Results : C. japonica administration significantly was protecting esophageal mucosal damage upon histological analysis of reflux esophagitis in rats. The C. japonica treatment confirmed the protection of the reduction of claudin-5, an evaluation index of the damage of tight junctions in the reflux esophagitis. C. japonica was also found to inhibit the expression of proteins such as COX-2 and TNF-α in the rat esophagus. C. japonica markedly attenuated the activation of NF-κB and phosphorylation of IκBα at the same time.
Conclusion : These results indicated that C. japonica suppressed the development of esophagitis through the modulation of inflammation by regulating NF-κB activation. Based on these findings, we concluded that C. japonica can prevent reflux esophagitis.
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KEYWORD
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Charybdis japonica, crab, reflux esophagitis, inflammation
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